I've got a feeling Sasuke gained more than just healing powers when he took Orochimaru's bijuu into his mind. For one thing, I have a really strong feeling he gained Orochimaru's ability to puke a snake/Orochimaru out. Which might mean that even if Itachi takes his eyes, a "new" Sasuke would appear, complete with eyes.
I don't see the point in Itachi taking his eyes now. In fact, it would ruin his "spares". Sasuke still doesn't have the Magnekyu Sharingan.