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The biggest thing I've seen is that anime has started to form even smaller subdivisions of itself, (mecha, shoujo, school life, ancient Japan (samurai, ninja), boys love, sci-fi, folklore specific, just to name a few), and I'm wondering which will die out and which will enjoy greater popularity (along with the changes and traditions institutionalized in each)...
I don't think of those as "subcultures", but more like subgenres within anime that will always exist. I also don't think any genre has shown itself to integrate into culture any more than the others (hell, look at the anime on American tv now, I'd say it's a pretty wide variety). What's really nice are the genre defining titles, that push themselves beyond any stereotypes or cookie-cutter plots. But, undoubtedly, there will always be shows that are exactly the opposite (we're still getting eva-clone mecha series, loads of harem shows, and epically long shounen adventures).